Tracking home quarantined people infected due to Corona virus using GPS & selfie and Detail about RT PCR Test

 

Tracking home quarantined people infected due to Corona virus using GPS & selfie




RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to the two other commonly used techniques for quantifying mRNA levels, Northern blot analysis and RNase protection assay, RT-PCR can be used to quantify mRNA levels from much smaller samples. In fact, this technique is sensitive enough to enable quantitation of RNA from a single cell.

This article first discusses the advantages of real-time RT-PCR compared to end-point methods. This discussion is followed by a description of the different methods for quantitating gene expression by real-time RT-PCR with respect to the different chemistries available, the quantitation methods used and the instrumentation options available. Subsequently, the “traditional” methods of quantitating gene expression by RT-PCR, i.e. end-point techniques, are presented.

 

Tracking home quarantined people infected due to Corona virus using GPS & selfie

While the coronavirus pandemic and climate change are inherently different issues, they share two important characteristics: both are global crises that threaten the lives of millions of people. As of Today the number of coronavirus infections approaches 100,000 people worldwide & increasing in India very rapidly.

In continuation to initiative taken by the Maharashtra government to reduce exposure of COVID-19, Suspected should undergo home quarantine for few weeks. These home quarantined people are supposed to stay home / hospital. Unfortunately many of them are not following these guidelines.

To stop people doing this, we have developed technological solution (Android application & website) to monitor home quarantined people with GPS system from corporation office itself.

Alongside to tracking, we can generate different reports which you need to share with health officials every day.

Surat Municipal Corporation(SMC) has taken various initiatives to fight COVID-19.

A team of developers at the Information Technology (IT) department of SMC has developed SMC COVID-19 Tracker System which includes a web portal and mobile application named “SMC COVID-19 Tracker” to track people who have abroad or interstate travel history and persons who have come in direct contact with positive COVID-19 individual.

Details of travelers and other individuals are collected through various sources like Self Declaration form on SMC website, calls received on helpline number, international traveler information received from Government of India(GoI), etc.

The brief about the functioning of the application is given below:

1. SMC has published Self Declaration Form on website www.suratmunicipal.gov.in where individuals can submit their details including their abroad or interstate travel history and if they have come in contact with any positive COVID-19 individual. Upon submission of details, an SMC is sent to individuals with unique Traveller ID and are asked to download SMC COVID-19 Tracker Mobile Application.

2. SMC has also started a helpline number 1-800-123-800 where a citizen can share details about travelers or suspects. The details are verified by the SMC team including health officials. A field team visits the location and if the details provided on the helpline is verified, the individual is asked to stay in home quarantine and they are also assigned unique Traveller ID and are asked to download SMC COVID-19 Tracker Mobile.

3. Individuals have to fill questionnaire twice a day (Morning 10 AM and Evening 9 PM) through SMC COVID-19 Tracker App regarding their health. After due consultation of health officials, we have prepared three questions in the questionnaire viz., if a person has fever, cough or difficulty in breathing. With this questionnaire, individual has to also send their Selfie (Photo). If any individual mentions issue in questionnaire regarding their health, initial follow up is done over the phone and if required, individuals are asked to visit nearby health facility for necessary check-up and treatment.

4. After successful installation of app, individuals have to send  their location every hour to confirm that they are following home quarantine on regular basis. SMC team monitors the location history of individuals if any individual is found not following the home quarantine guidelines, strict actions are taken against such individuals.

કોવિડ-19ની તપાસ:RT-PCR ટેસ્ટ શું છે, તે શા માટે કરવામાં આવે છે અને વિવિધ રાજ્યોમાં આ માટે કેટલો ચાર્જ વસુલવામાં આવે છે
એક દિવસ પહેલાલેખક: નિલેશ ઝીંઝુવાડીયા















Why Real-Time RT-PCR?

Over the last several years, the development of novel chemistries and instrumentation platforms enabling detection of PCR products on a real-time basis has led to widespread adoption of real-time RT-PCR as the method of choice for quantitating changes in gene expression. Furthermore, real-time RT-PCR has become the preferred method for validating results obtained from array analyses and other techniques that evaluate gene expression changes on a global scale.

To truly appreciate the benefits of real-time PCR, a review of PCR fundamentals is necessary. At the start of a PCR reaction, reagents are in excess, template and product are at low enough concentrations that product renaturation does not compete with primer binding, and amplification proceeds at a constant, exponential rate. The point at which the reaction rate ceases to be exponential and enters a linear phase of amplification is extremely variable, even among replicate samples, but it appears to be primarily due to product renaturation competing with primer binding (since adding more reagents or enzyme has little effect). At some later cycle the amplification rate drops to near zero (plateaus), and little more product is made.







What is RT-PCR test

RT-PCR (Reverse Transcription Polymers Chain Reaction) Test. In addition, rapid antigen and antibody tests are also done. If the rapid antigen test is positive, then the person is positive for corona. But if the test is negative and symptoms of corona still appear, it becomes necessary to have an RT-PCR test.

This test provides information about the virus in a person. In which the ribonucleic acid (RNA) of the virus is examined. Making RNA from deoxyribonucleic acid (DNA) is called transcription.


RT PCR TEST વિશે ડીટેઇલ માહિતી અહિંથી વાંચો

What is CT value

According to the Indian Council of Medical Research (ICMR), the RT-PCR test measures SRS-CoV-2 and then measures its positivity based on the cycle threshold (CT).

Samples need cycling several times to detect how much transition there is in the individual. In these circumstances if the CT value is 35 or less then the person appears to be infected. And if this value is less than 22 then hospitalization is required. CT values ​​23 to 39 are considered appropriate. Even in these circumstances a CT scan is advised to find out how widespread the infection is.

When the corona needs to be tested

Corona needs to be tested when there is fever, chest pain, odor and taste, difficulty breathing.

In addition, corona tests may be performed at a distance of 6 feet if in contact with an infected person or in contact with an infected person for more than 15 minutes.

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